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. 2016 Jun 1;6(6):1396–1407.

Figure 4.

Figure 4

Identification of ZEB1 as a direct target of miR-101. (A) The putative target genes of miR-101 were predicted using TargetScan, PicTar, and miRBase. (B) The predicted binding sites of miR-101 in the WT and MUT 3’-UTR of ZEB1. (C) Dual-luciferase reporter assays were performed 24 h after co-transfection of SCC-9 cells with miR-NC or miR-101 mimics and a pGL3 construct containing WT or MUT 3’-UTR of ZEB1. Data were normalized to those from cells co-transfected with miR-NC and pGL3 plasmid. The mRNA (D) and protein (E) levels of ZEB1 in SCC-9 cells transfected with miR-NC or miR-101 mimics were measured by qPCR and Western blot assays. GAPDH and β-actin were used as internal controls, respectively. All data are shown as mean ± SD of three separate experiments. **P < 0.01.