Figure 6.

Overexpression of miR-101 or knockdown of ZEB1 suppressed tumor growth and metastasis and promoted apoptosis of OSCC cells in vivo. SCID mice were injected subcutaneously or via their tail vein with SCC-9-luc cells that were infected with a control lentivirus (Lenti-pGCsi or Lenti-pLKO.1) or a recombinant lentivirus expressing a miR-101 precursor (Lenti-pGCsi-miR-101) or shZEB1 (Lenti-shZEB1). A. In vivo luciferase image for the detection of the xenograft tumor growth in the mice implanted with various cells. B. Tumor volume was measured and calculated once a week. C. Tumor weight was measured after six weeks of implantation. D. The numbers of metastatic foci in the lungs of mice from various groups eight weeks after tail vein injection. E. A TUNEL assay was performed to detect the percentage of apoptotic cells. All data are shown as mean ± SD of three separate experiments. *P < 0.05; **P < 0.01.