URI expression and transfection studies in gastric cancer cell lines. (A) Cells were harvested and lysed from gastric cancer cell lines SGC-7901, BGC-823, HGC-27, and MGC-803. Western blotting was performed. The basal expression of URI was shown in four gastric cancer cell lines. (B) Cells were harvested and lysed after transient transfection respectively with pCMV6-URI, URI siRNA-A, (B and C) for 48 hours in MGC-803 and HGC-27 cells, the expression of URI was detected by western blot, β-actin was used as an internal control. Left two panels: overexpression of URI; right two panels: knockdown of URI.