Figure 3.

URI inhibited apoptosis induced by adriamycin via caspase-dependent manner in MGC-803 and HGC-27 cells. After transfection of pCMV6-URI and URI siRNA-A for 48 hours, cells were treated with 250 nM adriamycin for 12 hours, apoptosis related protein Bax, Bcl-2, cleaved PARP-1, and cleaved caspase-3 were detected by western blotting. β-actin was used as an internal control. A and C. The relative value of Bax/Bcl-2 expression and the expression of cleaved caspase-3 and cleaved PARP-1 were significantly decreased in pCMV6-URI transfected cells compared with control cells. B and D. On the contrary, the expression of Bax/Bcl-2 and cleaved PARP-1 and cleaved caspase-3 were increased in URI siRNA-A transfected cells compared with scrambled control sequence and untransfected cells. E. After transfection of URI siRNA-A for 48 hours, cells were treated with 250 nM adriamycin for 12 hours. Apoptotic cells were detected by Annexin V/PI double staining via flow cytometer. The ratio of early apoptotic cells (Annexin V+/PI-) were significantly increased compared with control cells. A representative result from three independent experiments was shown. Values are expressed as means ± SD of three individual experiments. *P<0.05 or **P<0.01 relative to the control group.