FIGURE 6.

Porcine reproductive and respiratory syndrome virus-infected MØ are in tight contact with pDC during co-culture. MØ were infected with PRRSV Lena for 90 min followed by wash steps and then co-cultured with pDC during another 16 h. Then MØ cell membrane was labeled with GWA-AF633 (turquoise in A,B; brown in C,D), pDC surfaces were labeled with CD4-AF488 (green), and PRRSV N protein (red) was stained with SDOW17-A-AF546. 3D scans were acquired using confocal microscopy. (A,B) Two different types of the cell-to-cell contact were observed. All micrographs are 3D Blend images with threshold subtraction and gamma correction set as in mock-treated MØ. The images were acquired with zoom together with optimum resolution format to provide the best voxel size for each employed fluorochrome. (C,D) 3D images were created using the surface module of the Imaris software applied on the labeling of GWA-AF633 and CD4-AF488 for the MØ and the pDC surfaces, respectively.