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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Antiviral Res. 2016 Apr 6;131:19–25. doi: 10.1016/j.antiviral.2016.03.015

Figure 7. BRD3969 does not inhibit the RNA synthesis activity of the RSV polymerase in vitro.

Figure 7

Purified L-P complexes containing wt L protein (lanes 2–5) or an L protein variant (lane 1) were added to reaction mixtures containing either DMSO or varying concentrations of BRD3969 (serially diluted in DMSO) as indicated. The labeled products were separated by denaturing gel electrophoresis and visualized by autoradiography. Bands ≤ 25 nt in length represent products of de novo synthesis, bands of ≥26 nt in length represent products of back-priming and 3′ extension. The sizes of the RNA products were determined by comparison to an RNA ladder generated by alkali hydrolysis of a 32P end-labeled RNA oligonucleotide representing the anticipated RNA products. The positions of the 15, 20 and 25 markers are shown.