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Biochemical characterization of PlOMT4 in yeast cells by LC–MS analysis. (A), the conversion of the substrate 3′-hydroxy-daidzein to 3′-methoxy-daidzein (peak 1) by PlOMT4; (B), the mass spectra of the peak 1 with its authentic standard. WAT11 (pESC-HIS), the yeast strain carrying the empty vector pESC-HIS as a control; WAT11 (pESC-HIS-PlOMT4), the yeast strain expressing PlOMT4.
