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LC–MS analyses of the enzyme activities of the purified recombinant PlOMT4 in vitro. The enzyme activities of the recombinant PlOMT4 were shown for the conversion of 3′-hydroxy-daidzein to 3′-methoxy-daidzein (peak 1; A), the conversion of quercetin to isorhamnetin (peak 2; B), and the conversion of luteolin to chrysoeriol (peak 3; C). The mass spectra of the peaks 1–3 with their corresponding authentic standards were shown in Supplementary Figure S3.
