Skip to main content
. Author manuscript; available in PMC: 2016 Nov 30.
Published in final edited form as: Nat Struct Mol Biol. 2016 May 30;23(7):691–697. doi: 10.1038/nsmb.3237

Figure 5.

Figure 5

Spy conformation changes upon substrate binding. (a) Overlay of apo Spy (PDB ID: 3O39, gray) and bound Spy (green). (b) Overlay of WT Spy bound to Im76-45 (green), H96L Spy bound to Im7 L18A L19 AL13A (blue), H96L Spy bound to WT Im7 (yellow), and WT Spy bound to casein (salmon). (c) Competition assay showing Im76-45 competes with Im7 L18A L19A L37A H40W for the same binding site on Spy (further substrate competition assays are shown in Supplementary Fig. 8). Error bars depict standard deviations of n=3 technical replicates.