Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 23;124(7):957–965. doi: 10.1289/ehp.1509820

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, “Reproduced with permission from Environmental Health Perspectives”); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright.

PMC Copyright notice

Effects of developmental tetrachlorodibenzo-p-dioxin (TCDD) exposure on bone marrow hematopoietic progenitor cells after long-term competitive reconstitution. (A) c-Kit versus Sca1 flow cytometry plots from the primary vehicle-to-vehicle (V:V) competitive chimeras with CD45.1⁺lin^– cells in the left panel and CD45.2⁺lin^– cells in the right panel. The percentage of lin^– cells is specified by the number in each quadrant. (B) Flow cytometry plots from the primary vehicle to TCDD competitive chimeras. (C) Representative c-Kit versus Sca1 flow cytometry plots from the vehicle-to-vehicle secondary bone marrow chimera. (D) Flow cytometry plots from the secondary vehicle to TCDD (V:T) competitive chimeras. (E) Relative frequency of liⁿ–c-Kit⁺Sca1⁺ (LSK) hematopoietic progenitor cells in the CD45.2 population of cells compared with the CD45.1 competitor cells from the primary and secondary chimeras. White bars compare vehicle-to-vehicle chimeras, and blue bars compare the vehicle with TCDD chimeras. Solid bars are from the primary chimeras, and secondary chimeras are represented by bars containing slashes. The percentage of the control was calculated by dividing the frequency of c-Kit⁺Sca1⁺ cells in the CD45.2⁺lin^– population by the same population of CD45.1⁺lin^– cells. Data are the mean ± SEM from n = 5 chimeras, and the experiment was repeated once. *** Denotes statistically significant by analysis of variance (ANOVA) followed by Tukey’s test; p < 0.01 compared with the congenic cells from the same chimera.