Table 1.

ANOVA for variation in development time of 200 DGRP lines [degrees of freedom (DF); Type III mean squares (MS)]

Analysis	Reduced analyses	Source	DF	MS	Error DF	F-Value	p-Value	Variance component
Full model		Treatment	1	7012.292	188.68	422.94	< 0.0001	Fixed
		Sex	1	8.690	234.51	9.25	0.003	Fixed
		Sex × treatment	1	5.764	204.10	6.07	0.015	Fixed
		Line	200	47.754	185.92	2.68	< 0.0001	1.728
		Sex × line	199	0.941	185.76	0.99	0.531	0.003
		Line × treatment	187	17.953	187.18	18.87	< 0.0001	1.913
		Sex × line × treatment	183	0.952	2833.00	1.04	0.337	–0.019
		Residual	2,833	0.913				0.920
By treatment	Control	Sex	1	17.451	216.09	97.25	< 0.0001	Fixed
		Line	197	11.241	197.00	64.12	< 0.0001	1.148
		Sex × line	197	0.175	1536.00	0.49	1.000	0.000
		Residual	1,536	0.357				0.337
	Lead	Sex	1	0.104	234.47	0.06	0.802	Fixed
		Line	190	54.177	191.44	32.55	< 0.0001	6.198
		Sex × line	185	1.666	1297.00	1.06	0.287	0.017
		Residual	1,297	1.570				1.573
By treatment and sex	Control female	Line	197	5.568	769.00	15.50	< 0.0001	1.071
		Residual	769	0.359				0.359
	Control male	Line	197	5.963	767.00	16.77	< 0.0001	1.178
		Residual	767	0.356				0.356
	Lead female	Line	185	30.275	634.00	19.84	< 0.0001	6.682
		Residual	634	1.526				1.526
	Lead male	Line	190	26.592	663.00	16.48	< 0.0001	5.797
		Residual	663	1.613				1.614
h2 = (σ2L + σ2L × S + σ2L × T + σ2L × T × S)/(σ2L + σ2L × S + σ2L × T + σ2L × T × S + ε) = 0.76.