Table 3.

ANOVA for adult activity of 166 DGRP lines [degrees of freedom (DF); Type III mean squares (MS)]

Analysis	Reduced analyses	Source	DF	MS	Error DF	F-Value	p-Value	Variance component
Full model		Treatment	1	739980.1	170.1	0.75	0.3881	Fixed
		Sex	1	275320.6	167.7	0.15	0.6989	Fixed
		Treatment × sex	1	253499.5	173.6	0.43	0.5144	Fixed
		Line	165	20507687.5	176.0	8.76	< 0.0001	121983.4
		Sex × line	165	1923507.8	165	3.14	< 0.0001	18092.6
		Treatment × line	165	1029336.1	165	1.68	0.0005	5230.5
		Treatment × sex × line	165	612337.5	25,600	2.24	< 0.0001	9218.6
		Residual	25,600	273954.7	170.1			273941.6
By treatment	Control	Sex	1	603134.9	167.31	0.43	0.5121	Fixed
		Line	165	11822085.7	165	8.18	< 0.0001	121949.8
		Sex × line	165	1445867.5	13,764	5.98	< 0.0001	27771.5
		Residual	13,764	241671.0				241654.6
	Lead	Sex	1	200.4	176.4	0.00	0.9894	Fixed
		Line	165	10688962.1	165	8.64	< 0.0001	132659.8
		Sex × line	165	1236961.0	11,836	3.97	< 0.0001	26293.8
		Residual	11,836	311497.2	167.3			311485.3
By treatment and sex	Control female	Line	165	6368290.1	6,874	27.93	< 0.0001	141348.8
		Residual	6,874	228037.9				228030.5
	Control male	Line	165	6941945.0	6,890	27.19	< 0.0001	157929.9
		Residual	6,890	255272.5				255270.7
	Lead female	Line	165	5769243.4	5,884	25.72	< 0.0001	146230.3
		Residual	5,884	224273.1				224276.7
	Lead male	Line	165	6668344.8	5,952	16.77	< 0.0001	168890.3
		Residual	5,952	397724.8	6,874			397735.5
Twenty-four lines were excluded from this analysis because of low yield of adult flies. h2 = (σ2L + σ2L × S + σ2L × T + σ2L × T × S)/(σ2L + σ2L × S + σ2L × T + σ2L × T × S + ε) = 0.36.