Table 1.

Primer names and sequences used for the real-time PCR of M. × domestica CalS genes.

Primer name	Sequence (5′ → 3′)
MdCalS1 Fwd	CGGTCGCCTTTATCTTGTTC
MdCalS1 Rev	TAACCATAGGCAAGGCCATC
MdCalS2 Fwd	ACCTAATCCAGTGGCCTCCT G
MdCalS2 Rev	CGATCTCCAAGGACCAAAAA
MdCalS3-A Fwd	TCTTGCTTTCATGCCAACTG
MdCalS3-A Rev	TGCTGAATGCTTGGTTGAAG
MdCalS3-B Fwd	TGAGATTCATGCCAGAGTGC
MdCalS3-B Rev	AAGCAACCAGAAACCACAGG
MdCalS5-A Fwd	TAATTCAGTGGCCACCCTTC
MdCalS5-A Rev	ATCTGCACATATCCGCTTCC
MdCalS5-B Fwd	CTTTGAAGATCCGCTTCCAC
MdCalS5-B Rev	TGTCCTCGCTCAGATTGATG
MdCalS7-A Fwd	TCTACGGGCAATCCTTTGAC
MdCalS7-A Rev	CCAATACCACCACGATTTCC
MdCalS7-B Fwd	TGGAAGTGCTGATTCTGCTG
MdCalS7-B Rev	AAGCAACCAGAAACCACAGG
MdCalS8 Fwd	AATCCTCGTTGTTGGTCGTC
MdCalS8 Rev	GACCTTGCGATGCTTTCTTC
MdCalS9 Fwd	TGTGTACGGCTTCTGCATTC
MdCalS9 Rev	TTGAGCACTGTGCAAACCTC
MdCalS11 Fwd	TGTTCAATGAAGCGTTCAGC
MdCalS11 Rev	CGAAAAGATTCGGAGACTCG
MdCalS12 Fwd	GCTTTTGGCATTTTTGTGGT
MdCalS12 Rev	ACAGGGCCGAAATACTTGTG
Ubq Fwd	TGATCTTCGCTGGAAAACAG
Ubq Rev	CCTGAATTTTTGCCTTGACG
GAPDH Fwd	GTTCGTTGTTGGTGTGAACG
GAPDH Rev	GTCTTCTGGGTGGCAGTGAT