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12Z preferentially adhered to laminin and collagen IV, two key components of the basement membrane, with lower levels of adhesion to collagen I and fibrin. 12Z were plated in serum-free media for 2 hours on adsorbed ECM proteins, stained with the nuclear stain TO-PRO-3, and the number of adherent cells was quantified via fluorescence intensity. Cells that were plated in complete media were used as a positive control and wells coated with 1% BSA were used as a negative control.
