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Imaging bone with second harmonic generation (SHG) microscopy. Collagen fibril bundles from porcine cortical bone (in green in the online version). A grid has been superimposed in order to compartmentalize the picture that enables semi-quantitative assessment of the 2D orientation of the collagen fibrils (white arrows). (Image from [205] with kind permission of Elsevier.) (Online version in colour.)
