Figure 13.

Imaging bone with transmission electron microscopy (TEM). (a–d) Bone ultrastructure arrangement of decalcified trabecular (a,b) and cortical (c,d) rat tibiae. Collagen arrangement in bones of control animals (a,c) in qualitative comparison with bones from ovariectomized animals (b,d). (Images from [233] with kind permission of Elsevier.) (e) Single mineralized collagen fibril, where the approximately 67 nm D-spacing of collagen is visible and can be measured using Fourier transform of the image (inset). (Image from [7] with kind permission of American Chemical Society Publications.)