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Imaging bone with serial focused ion beam scanning electron microscopy (FIB SEM). (a–d) Four images out of a 3D stack from demineralized rat tibiae (cortical bone), imaged using serial FIB SEM at a (lateral) pixel size and a slice thickness of about 10 nm. Fast Fourier transform is performed to assess the ultrastructure orientation in two dimensions (insets). (Images from [208] with kind permission of Elsevier.)
