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. 2016 Jun;13(119):20160088. doi: 10.1098/rsif.2016.0088

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© 2016 The Author(s)

Published by the Royal Society. All rights reserved.

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Figure 15. — Imaging bone with atomic force microscopy (AFM). (a–c) Murine cortical bone. (Images from [253] with kind permission of Elsevier.) (a) Polished cortical bone surface, mineralized. (b) Cortical bone surface demineralized using ethylenediaminetetraacetic acid (EDTA). The collagen fibrils are nicely exposed. (c) Collagen fibrils corresponding to the inset area in (b). The characteristic D-spacing as well as their 2D organization are clearly visible. (d–f) D-spacing measurements of collagen fibrils of ovariectomized sheep dermis. (Images from [263] with kind permission of Wiley-VCH Verlag GmbH & Co.) (Online version in colour.)