Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun;13(119):20160088. doi: 10.1098/rsif.2016.0088

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Author(s)

Published by the Royal Society. All rights reserved.

PMC Copyright notice

Figure 9. — Imaging bone with confocal laser scanning microscopy (CLSM). (a) CLSM image of a single lamella from a human femur. The arrow represents a qualitative assessment of the orientation of the collagen fibrils. (Image from [63] with kind permission of Elsevier.) (b,c) Difference in the focusing capabilities of CLSM versus multi-photon microscopy. (Images from [191] with kind permission of Nature Publishing Group.) (b) In the case of CLSM, the laser beam excites molecules outside of the focal plane on its path through the tissue. (c) In multi-photon microscopy, (at least) two photons are combined to specifically excite only the molecules at the focal spot. (Online version in colour.)