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. Author manuscript; available in PMC: 2016 Oct 9.
Published in final edited form as: J Mol Biol. 2015 Aug 21;427(20):3316–3326. doi: 10.1016/j.jmb.2015.08.014

Figure 3.

Figure 3

Characterization of functional MarA variants. A) Class 1 variants exhibit wild type-like growth on plates following a 24-hour incubation when transformed into E. coli. Class 2 variants also result in numerous colonies but require a 48-hour incubation. Class 3 variants exhibit the least robust growth, resulting in very few colonies regardless of incubation time. B) Mean log-phase growth rates of E. coli transformed with the variant indicated. Standard errors for wild type, Y109F, and I68V/L94M replicates were 0.033, 0.051, and 0.011, respectively. Standard error among all growth rates was 0.025. C) Increasing concentrations of wild type or L72I/Y109F (class 3) MarA were mixed with radioactively labeled target DNA, equilibrated for 30 minutes, and passed through Tuffryn size exclusion membrane. Percentages indicate amount of total signal present on the Tuffryn membrane.