TABLE 2.

NHEJ editing efficiency and embryo viability under multiple CRISPR-EZ conditions

Gene	CRISPR-EZ conditions	Zygotes treated	Embryos survived to morula	Embryos assayed by RFLP	Editing
					Bi-allelic	Partial	Unedited
Tyr	16 μm Cas9 RNPs, 1 ms	29	8 (28%)	8	1 (13%)	7 (87%)	0
	16 μm Cas9 RNPs, 3 ms	24	6 (25%)	6	5 (83%)	1 (17%)	0
	16 μm Cas9 RNPs, 10 ms	28	5 (18%)	5	5 (100%)	0	0
	8 μm Cas9 RNPs, 1 ms	30	19 (63%)	12	2 (16%)	9 (75%)	1 (8%)
	8 μm Cas9 RNPs, 3 ms	30	18 (60%)	12	8 (67%)	4 (33%)	0
	8 μm Cas9 RNPs, 10 ms	30	12 (40%)	12	11 (92%)	1 (8%)	0
Cdh1	8 μm Cas9 RNPs, 3 ms	35	NDa	25	0	14 (56%)	11 (44%)
Cdk8	8 μm Cas9 RNPs, 3 ms	35	ND	22	3 (14%)	19 (86%)	0
Kif11	8 μm Cas9 RNPs, 3 ms	35	ND	24	1 (4%)	12 (50%)	11 (46%)

^a ND means not determined.