FIGURE 9.

Immunofluorescence staining for WT1 and nephrin in anti-GBM nephritis. A and B, iPLA₂γ deletion reduced podocyte number in anti-GBM nephritis. WT1 immunofluorescence was assessed in two WT mice without and with anti-GBM antibody and four iPLA₂γ KO mice (age 3–4 months), without and with anti-GBM antibody. Bar, 20 μm. B, quantification of WT1-positive nuclei per glomerulus (25–36 glomeruli/group). *, p < 0.001 KO/anti-GBM versus WT/anti-GBM. A and C, nephrin immunofluorescence intensity was assessed in iPLA₂γ KO mice without (n = 2) and with anti-GBM antibody (n = 6) and in WT mice without (n = 2) and with anti-GBM antibody (n = 5). *, p < 0.0001 anti-GBM versus saline (14–31 measurements/group). Error bars, S.E.