TABLE 2.
Kinetics of ATP hydrolysis
| Enzymes | Nucleic acid-stimulated ATPase |
Basal ATPase |
||
|---|---|---|---|---|
| kcata | KNAb | kcata | KATPc | |
| WT | 822.4 ± 51.1 | 47.4 ± 6.8 | 32.29 ± 4.60 | 0.14 ± 0.06 |
| WT+BMDB | 882.4 ± 99.3 | 65.2 ± 14.7 | 33.01 ± 5.35 | 0.11 ± 0.05 |
| CCi | 776.2 ± 51.9 | 55.1 ± 8.0 | 31.82 ± 4.71 | 0.13 ± 0.06 |
| CCi+BMDB | 794.1 ± 42.9 | 43.2 ± 5.6 | 32.99 ± 6.69 | 0.10 ± 0.05 |
| CC1 | 683.7 ± 41.2 | 40.0 ± 6.0 | 32.11 ± 4.06 | 0.12 ± 0.05 |
| CC1+BMDB | 125.5 ± 17.1 | 73.9 ± 19.2 | 8.79 ± 0.41 | 0.80 ± 0.15 |
| CC2 | 697.7 ± 69.6 | 42.9 ± 10.4 | 32.57 ± 6.47 | 0.11 ± 0.06 |
| CC2+BMDB | 268.5 ± 23.6 | 35.9 ± 8.3 | 13.73 ± 1.99 | 0.13 ± 0.06 |
| CC1 | 615.0 ± 49.3 | 33.7 ± 7.3 | ||
| CC1+NEM | 556.7 ± 29.6 | 29.6 ± 4.5 | ||
| CC2 | 627.3 ± 26.8 | 28.7 ± 3.5 | ||
| CC2+NEM | 678.3 ± 41.6 | 44.8 ± 6.5 | ||
a Values are maximum turnover rates (pmol ATP hydrolyzed/min/pmol of protein).
b Values are concentrations of dT12 (μm) at 50% of the maximum velocities.
c Values are concentrations of ATP (mm) at 50% of the maximum velocities.