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. 2016 May 5;291(28):14540–14554. doi: 10.1074/jbc.M115.711267

FIGURE 4.

FIGURE 4.

LC-MS resolution of linoleate triol standards as the PFB ester DMP derivative. A, four triol hydrolysis products (triol-1 to -4) of 9R,10R-epoxy-13R-hydroxyoctadec-11E-enoic acid are analyzed as the PFB ester DMP derivative using a Thomson Advantage Silica 150Å 5-μ, column (25 × 0.46 cm) with a solvent of hexane/IPA 100/1 (by volume) at a flow rate 1 ml/min, with UV detection at 205 nm. B, in autoxidized d4 epoxy alcohol, the same four products were chromatographed with the triols (PFB ester DMP derivative) from the second major epoxy alcohol of the hematin reaction, the 13S diastereomer, 9R,10R-epoxy-13S-hydroxyoctadec-11E-enoate (triol-5 to -8).