FIGURE 5.

Comparison of ECCE in FDB fibers. Upper panel, fura-2 manganese quenching was carried out as described under “Experimental Procedures.” Values are the mean ± S.D. n indicates the number of FDB fibers investigated. *, multiple comparison Dunnett's ANOVA post test: DKO1 versus WT, p < 0.01; DKO1 versus CASQ2 KO, p < 0.01; DKO1 versus CASQ1 KO, p < 0.01; DKO1 versus JP45 KO, p < 0.01; TKO versus WT, p < 0.01; TKO versus CASQ2 KO, p < 0.01; TKO versus CASQ1 KO, p < 0.01; TKO versus JP45 KO, p < 0.01. °, multiple comparison Dunnett's ANOVA post test: DKO2 versus WT, p < 0.05; DKO2 versus CASQ1 KO. p < 0.05. Lower panel, fura-2 manganese quenching was carried out as described under “Experimental Procedures.” Single fibers were perfused with Tyrode's solution in which 1.8 mm CaCl₂ was replaced with manganese and then stimulated by a 300-ms duration train of pulses delivered at 100 Hz. The red and black traces represent recordings carried out in the absence and in the presence of 50 μm nifedipine in the extracellular buffer, respectively. Representative traces of experiments of fibers isolated from four to six mice of each genotype are shown.