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. 2016 May 4;291(28):14555–14565. doi: 10.1074/jbc.M115.709071

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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FIGURE 7. — Effect of high ionic strength on JP45-CASQs isoforms interaction. Pulldown assays were carried out as described Fig 6, panels A and B. Solubilization of membrane fraction proteins and interaction with GST-JP45 fusion protein was carried out as described under “Experimental Procedures” in the presence of 1 m NaCl. V, void; LW, last wash; B, bound to the GST-JP45 fusion protein glutathione-Sepharose beads. Shown are representative immunoblots of three experiments carried out with two different total SR preparations. Left panel, staining of the blot membrane with anti-CASQ1 Ab. Right panel, staining of the blot membrane with anti-CASQ2 Ab. TC, skeletal muscle terminal cisternae fraction.