FIGURE 3.

F1L is not able to prevent caspase-mediated yeast cell death. A, reconstitution of the caspase activation pathway (Apaf-1, caspase-9, and caspase-3) in S. cerevisiae. Yeast were co-transformed with constructs encoding Apaf-1, caspase-9, and caspase-3 and the indicated apoptosis regulatory proteins or empty vector, each under the control of an inducible (GAL) promoter. 5-Fold serial dilutions were spotted onto inducing galactose or repressing glucose plates. Colony size indicates growth rate, and colony number reflects cell viability. Each dilution was also spotted onto a control plate (glucose) to verify that equivalent numbers of each transformant were spotted. B, yeast co-transformed with constructs encoding Bax or Bak and the indicated prosurvival proteins, each under the control of an inducible (GAL) promoter, were spotted onto inducing galactose or repressing glucose plates as 5-fold serial dilutions. A and B, images are representative of two independent experiments. White spaces indicate where an irrelevant lane was spliced out of the plate photographs.