FIGURE 2.

Purification of recombinant chicken BCO2 using cobalt affinity column chromatography. The SDS-polyacrylamide gel on the left shows the elution of recombinant His-tagged human BCO2 (theoretical mass, 64,202 Da) from the cobalt column by increasing concentrations of imidazole. Only the fractions that show a single band (lanes 5–7) were combined and desalted into the final storage buffer. The SDS-polyacrylamide gel on the right shows the combined fractions and the protein obtained after desalting.