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Substrate velocity plot for chicken BCO2 with β-cryptoxanthin at 37 °C. A plot of reaction velocity as a function of β-cryptoxanthin concentration using 1 μg of BCO2/200-μl reaction and a 30-min reaction time is shown. The amounts of 3-hydroxy-β-apo-10′-carotenal and 3-hydroxy-β-apo-8′-carotenal were estimated using β-apo-10′-carotenal and β-apo-8′-carotenal as standards. Each data point represents the average of duplicate experiments.
