TABLE 1.

Sequences of oligonucleotide primers used to mutate full-length and truncated (57–678) CYPOR

Mutant	Sequence
ΔGly-141 (forward)	5′-TGC ATG GCC ACA TAC—GAG GGC GAC C-3′
ΔG141/E142N (forward)	5′-TGC ATG GCC ACA TAC—AAC GGC GAC C-′
ΔGly-143 (forward)	5′-ATG GCC ACA TAC GGA GAG—GAC CCC A-3′
Stop codon (forward)	5′-GCC ACT GGG GAG GAG TAG TAG ATT C-′