TABLE 7.

Kinetics of reduction of wild-type CYPOR and glycine deletion mutants by 10 molar eq of NADPH

Reductase	λ/nm	A1 (%)	k1	A2 (%)	k2
			s−1		s−1
WT CYPOR	390	50.2 ± 0.1	66.7 ± 1.3	49.8 ± 0.1	6.1 ± 0.1
	452	67.3 ± 1.0	60.7 ± 1.7	32.7 ± 1.0	6.4 ± 0.4
	590		54.9 ± 3.3		7.2 ± 0.8 (Decay)
ΔGly-141	390				7.8 ± 0.5 (Decay)
	466	36.5 ± 0.7	82.4 ± 4.6	63.5 ± 0.7	7.9 ± 0.2
	590		73.6 ± 3.2		14.7 ± 0.4 (Decay)
ΔG141/E142N	390	19.1	40.1 ± 2.3	68.98	7.5 ± 0.2 (Decay)
	466	56.5 ± 0.4	80.6 ± 2.5	43.5 ± 0.4	5.4 ± 0.1
	590		66.8 ± 1.8		6.8 ± 0.2 (Decay)
ΔGly-143	390	40.2	60.6 ± 2.5	59.8	7.3 ± 0.4 (Decay)
	459	43.5 ± 1.4	61.5 ± 2.7	56.5 ± 1.4	8.4 ± 0.2
	590		41.9 ± 2.0		5.8 ± 0.2 (Decay)