FIGURE 1.

Cloning of Rab5a homolog from L. donovani. A, amplification of 708-bp (lane 2) fragment of Ldrab5a from L. donovani cDNA by PCR. B, alignment of the amino acid sequence of LdRab5a with Rab5a sequences from different organisms. Residues implicated in guanine phosphate binding (PM1–3), GTP/GDP binding (G1–3), and the isoprenylation motif (I) are marked in bold. The sequence of LdRab5a has been submitted to the GenBank^TM database under accession number KP308372. C, comparison of LdRab5a with LdRab5b sequences. Sequence alignment of LdRab5a and LdRab5b shows the presence of highly conserved regions of Rab proteins like the guanine nucleotide-binding regions (G1–G3), phosphate/Mg²⁺ (PM1–PM3) regions, and the C-terminal isoprenylation motif. Five Rab family (RabF1–RabF5) motifs are marked in blue, and Rab5-specific subfamily motifs (SF1–SF4 regions) are indicated in red.