FIGURE 2.

Specificities of anti-LdRab5a and anti-LdRab5b antibodies. A, specificities of anti-LdRab5a and anti-LdRab5b antibodies were determined using purified His₆-LdRab5a and His₆-LdRab5b proteins by Western blot analysis. B, Western blot analysis was carried out using lysates from LdRab5a- or LdRab5b-overexpressing Leishmania by anti-LdRab5a and anti-LdRab5b antibodies (left panel). Purified endosome containing avidin-HRP or biotinylated-Hbs were probed with anti-LdRab5a or anti-LdRab5b antibodies, respectively (right panel). All results are representative of three independent observations.