FIGURE 8.

Identification of putative effectors of LdRab5 isoforms by mass spectrometry. A, to identify the LdRab5a- and LdRab5b-interacting partners, immobilized GST-LdRab5a or GST-LdRab5b was incubated with Leishmania lysate, and proteins bound with respective LdRab5 were analyzed by mass spectrometry as described under “Experimental Procedures.” Venn diagram shows the number of proteins from Leishmania bind with indicated LdRab5. Results are representative of three independent experiments. B, binding of immobilized GST-LdRab5a and GST-LdRab5b with LdCHC or HbR from GFP-LdCHC-overexpressing Leishmania lysate as described under “Experimental Procedures.” Western blot analysis was carried out using anti-GFP and anti-HbR antibodies. All results are representative of three independent observations.