FIGURE 3.

Differential regulation of Tmc1 and canonical proteasome components by Rpn4. A, relative protein abundance of Tmc1 and the proteasome subunits Rpn7, Pre5 (Alpha6), and Pre1 (Beta4) after treatment with sodium arsenite (1 mm) for the indicated times. B, results for Rpn4. The data were generated using a proteome-wide TMT-based mass spectrometry approach (11). The error bars represent standard deviations from triplicate cultures. In addition, all differences between untreated and treated samples were statistically significant by Student's t test (p < 0.02).