FIGURE 5.

Proteostasis-related phenotypes of Tmc1. A, growth of wild-type, tmc1Δ, and hcr1Δ strains in the presence or absence of sodium arsenite (2 mm), as indicated. The cells were spotted in 3-fold serial dilutions and cultured at 30 °C for 2 days. B, growth of wild-type cells expressing an empty vector or a TMC1 overexpression vector in the presence of sodium arsenite (1.75 mm) or cycloheximide (0.25 μg/ml), as indicated. The cells were spotted in 3-fold serial dilutions and cultured at 30 °C for 2–6 days. Duplicate strains are shown. C, growth of wild-type and hcr1Δ strains expressing an empty vector or a TMC1 overexpression vector in the presence or absence of cycloheximide (0.25 μg/ml), as indicated. The cells were spotted in 3-fold serial dilutions and cultured at 30 °C for 2–7 days. D, growth of wild-type and rpl39Δ strains expressing an empty vector or a TMC1 overexpression vector in the presence or absence of cycloheximide (0.25 μg/ml), as indicated. The cells were spotted in 3-fold serial dilutions and cultured at 30 °C for 2–7 days.