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Diverse proteotoxic stresses induce Tmc1. Tmc1-TAP protein levels after treatment with cadmium chloride (A) or canavanine (B) at the indicated concentrations for 1 h. Whole cell extracts were prepared and analyzed by SDS-PAGE followed by immunoblot. Upper panel, anti-TAP antibody. Lower panel, anti-Pgk1 antibody (loading control).
