TABLE 1.
Yeast strains
Strains from the Research Genetics Collections (RGC) are available through ThermoFisher Scientific.
| Name | Genotype | Source |
|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | RGC |
| Tmc1-TAP | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TMC1::TMC1-TAP (HIS3) | RGC |
| Tmc1-GFP | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TMC1::TMC1-GFP (HIS3) | RGC |
| rpn4Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rpn4::KAN | RGC |
| yap1Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 yap1::KAN | RGC |
| yap8Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 yap8::KAN | RGC |
| sGM39 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TMC1::TMC1-TAP (HIS3) rpn4::KAN | This study |
| sGM14 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TMC1::TMC1-TAP (HIS3) pdr5::KAN | This study |
| rad6Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad6::KAN | RGC |
| ubc4Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubc4::KAN | RGC |
| ubc5Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubc5::KAN | RGC |
| ubc7Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubc7::KAN | RGC |
| ubc8Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubc8::KAN | RGC |
| ubc11Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubc11::KAN | RGC |
| ubc13Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubc13Δ::KAN | RGC |
| tmc1Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tmc1::KAN | RGC |
| hcr1Δ | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 hcr1::KAN | RGC |
| sGM2 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 [pKT10] | This study |
| sGM1 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 [pGM1] | This study |
| sJH481 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 hcr1::KAN [pKT10] | This study |
| sJH482 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 hcr1::KAN [pGM1] | This study |
| sGM95 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rpl39::KAN [pKT10] | This study |
| sGM96 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rpl39::KAN [pGM1] | This study |
| sUB62 | MATa lys2-801 leu2-3, 2-112 ura3-52 his3-200 trp1-1 | Ref. 29 |
| sUB453 | MATa lys2-801 leu2-3, 2-112 ura3-52 his3-200 trp1-1 ubc4::HIS3 ubc5::LEU3 | Ref. 30 |