FIGURE 5.

Overexpression of integrin αv and β1 enhances the fusogenic activity of the aMPV/B F protein and the replication of aMPV/B. A–D, cells were transfected with plasmid DNAs expressing integrin αv and β1 from different species. aMPV/B F protein fusogenic activities were determined by syncytium formation and reporter gene assays. Ma, monkey; Mo, mouse; Ch, chicken. E, G, and I, the kinetics of aMPV/B replication in different cells overexpressing integrin αv and β1 were determined up to 96 h.p.i. F, H, and J, at 72 h.p.i., the supernatant was collected from infected Vero, BHK-21, or DF-1 cells to determine the virus titer and compared with controls. The virus titer of transfection with the vector pCAGGS was set as 100%. All experiments were repeated three to five times. p < 0.05 and p < 0.005 are indicated by * and **, respectively.