FIGURE 8.

CS/DS from HCC70 cells primed on either XylNapOH or XylNap have a cytotoxic effect, but HS from HCC70 cells primed on XylNap inhibits this effect. HCC70 cells were treated with xyloside-primed GAGs from HCC70 cells for 96 h. A and B, effect on growth by XylNapOH-primed GAGs (A) or XylNap-primed GAGs (B), untreated (white circles) or heparinase I-III-treated (gray squares) or chondroitinase ABC-treated (black circles) (R² = 0.97, 0.88, and 0.98 for the curves of the untreated and heparinase I-III-treated XylNapOH-primed GAGs and heparinase I-III-treated XylNap-primed GAGs from HCC70 cells, respectively). C, heparinase I-III-treated XylNapOH-primed GAGs together with chondroitinase ABC-treated XylNap-primed GAGs, both at increasing concentrations. The untreated XylNapOH- and XylNap-primed GAGs were administered at the indicated concentrations, whereas the enzyme-treated GAGs were diluted in the same way as the untreated ones before administration. D, fixed concentration (7.5 μg/ml) of heparinase I-III-treated XylNapOH-primed GAGs combined with the indicated concentrations of chondroitinase ABC-treated XylNap-primed GAGs. The graphs are representative for each experiment, performed at least in duplicate, in which n = 3. The data points are the means ± S.D.