Table 2. Internalization of silica nanospheres by bacterial and yeast cells after EMF exposures.
| Silica nanospheres | ||||
|---|---|---|---|---|
| 23.5 nm | 46.3 nm | |||
| Bacterial strains | Loading capacity * | Amount of cells took up nanospheres(%) | Loading capacity * | Amount of cells took up nanospheres (%) |
| Branhamella catarrhalis ATCC 23246* | 183 ± 8 | 98 ± 4 | Not detected | Not applicable |
| Kocuria rosea CIP 71.15T* | 139 ± 8 | 99 ± 5 | 62 ± 8 | 83 ± 8 |
| Planococcus maritimus KMM 3738 | 172 ± 8 | 97 ± 5 | 75 ± 8 | 80 ± 9 |
| Staphylococcus aureus ATCC 25923 | 161 ± 8 | 99 ± 4 | 81 ± 8 | 40 ± 7 |
| Staphylococcus aureus CIP 65.8T | 261 ± 8 | 99 ± 3 | 114 ± 8 | 44 ± 7 |
| Staphylococcus epidermidis ATCC 14990T | 211 ± 8 | 99 ± 5 | Not detected | Not applicable |
| Streptomyces griseus ATCC 23915* | 200 ± 8 | 99 ± 5 | 109 ± 8 | 55 ± 8 |
| Saccharomyces cerevisiae ATCC 287* | 27778 ± 8 | 97 ± 5 | Not detected | Not applicable |
* per single cell
Nanosphere loading capacity was calculated using the fluorescence intensity of nanospheres. The number of bacterial and yeast cells that were able to internalize the nanospheres, expressed as a percentage, was calculated by counting fluorescent cells in the CLSM images. Data are means ± SD and are representative of 3 independent experiments.