Table 1. Primer sequences of genes analyzed by real-time PCR.
Name | Accession number | Sense (5'---3') | Antisense (5'---3') |
---|---|---|---|
β-actin | NM_031144.3 | GGCATCCTGACCCTGAAGTA | GGGGTGTTGAAGGTCTCAAA |
SOD2 | NM_001270850.1 | AGCTGCACCACAGCAAGCAC | TCCACCACCCTTAGGGCTCA |
CAT | NM_012520.2 | TCCGGGATCTTTTTAACGCCATTG | TCGAGCACGGTAGGGACAGTTCAC |
GPx | NM_017006.2 | CGGTTTCCCGTGCAATCAGT | ACACCGGGGACCAAATGATG |
Nrf2 | NM_031789.2 | GGTTGCCCACATTCCCAAAC | GGCTGGGAATATCCAGGGC |
HO-1 | NM_012580.2 | GCGAAACAAGCAGAACCCA | GCTCAGGATGAGTACCTCCCA |
Bcl-2 | NM_016993.1 | CTGGTGGACAACATCGCTCTG | GGTCTGCTGACCTCACTTGTG |
Bax | NM_017059.2 | GGCGAATTGGCGATGAACTG | ATGGTTCTGATCAGCTCGGG |
SOD2: Manganese-dependent superoxide dismutase (MnSOD); CAT: Catalase; GPx: Glutathione peroxidase; Nrf2: Nuclear factor erythroid 2–related factor 2; HO-1: Heme oxygenase 1; Bcl-2: B-cell lymphoma 2; Bax: Bcl-2-like protein 4.