Fig 3. Illustration of how the mean isotopic ratios of δ¹⁵N and δ¹³C change when different quantities of mosquitoes from enriched versus control basins are included in the pools for analysis.

Mean (CI: 95%) δ¹⁵N and δ¹³C for mosquitoes obtained from basins that were either enriched using the stable isotopes (enriched mosquitoes) or control basins that were not enriched (unenriched mosquitoes). We analysed adult mosquitoes in pools containing a total of four mosquitoes, but variable ratios of enriched to unenriched mosquitoes as indicated by the x-axis label (i.e. 0/4, 1/4, 2/4, ¾ or 4/4). Panel A represents females for Aedes aegypti and Anopheles gambiae sensu lato, while panel B represents males of the same species. All values referenced against international standards (nitrogen = air; carbon = Vienna Pee Dee Belemnite (VPDB)).