Figure 3.

I.t.-delivered rictor^−/− DC show similar migration to draining lymphoid tissue, but reduce the frequency of MDSC within the tumor. 5 × 10⁶ CFSE-labeled control DC or Rictor^−/− DC were injected i.t. on day 7 post-tumor inoculation in B16-melanoma-bearing B6 mice. After 3 d, tumors, spleens and tumor-draining inguinal lymph nodes were harvested and cells isolated. (A) Plots show the percentages of CFSE⁺ DC recovered from the tumor. (B, C) Absolute numbers of CFSE⁺ DC recovered from the tumor (B) and inguinal lymph nodes (C). Box plots show median, 25%- and 75%-quartiles, and both extreme values. (D) Percent CD11c⁺ and CD11b⁺Gr1⁺ cells in the tumor shown as means + SD for three animals per group. *p < 0.05.