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. 2016 Apr 22;5(6):e1166323. doi: 10.1080/2162402X.2016.1166323

Figure 1.

Figure 1.

rED44Her2 and rED44Her2-FrC conjugate vaccines induce potent humoral immunity against rED44Her2 and native rHer2, and protect against challenge with the TUBO mammary carcinoma. (A) Schematic representation of the Her2 molecule (highlighting the ED44Her2 fragment) and the ED44Her2-FrC conjugate vaccine design. Diagram is representative of both the rat and human forms of Her2 and ED44Her2. (B) BALB/c wild type mice were primed and boosted 3 weeks later with rED44Her2, rED44Her2-FrC or the plant expressed control vaccine, all in alum, or the EC-TM DNA vaccine (n = 5 per group). Antibody specific for the rED44Her2 fragment was measured by ELISA. Serum was collected after priming, at week 3, and at week 5 which was 2 weeks after the boost. (C) Antibody able to bind native rHer2 expressed on the surface of TUBO cells was measured by flow cytometry at week 5 and quantified using internal standards. (D) Correlation between the levels of anti-rED44Her2 antibody, as measured by ELISA, and anti-rHer2 antibody, as measured by binding to native rHer2 at the cell surface. Data from the rED44Her2 vaccine at week 5. r = the Spearman's rank correlation coefficient. (E) 6 weeks after the booster injection (week 9) mice were challenged with the transplantable TUBO mammary carcinoma, and culled when tumors reached 15 mm diameter. In (B) and (C) each bar represents medians with individual mice displayed as dots. Mann–Whitney statistics are shown. Log-rank (Mantel–Cox) test results shown in (E). ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. For (B) and (D) data from two experiments has been combined. For (C) and (E) one representative experiment of three is shown.