Figure 7.

Alloreactive CD4⁺ and CD8⁺ iTregs display differential gene expression profiles. Naive CD4⁺ or CD8⁺ B6-Foxp3-GFP T cells were stimulated with BALB/c CD11c^hi DC with IL-2 only, or with TGFβ and RA three separate times. GFP/Foxp3⁻ CD4⁺ or CD8⁺ cells were FACS sorted from the culture with IL-2 alone, and GFP/Foxp3⁺ CD4⁺ or CD8⁺ cells were sorted from the culture with IL-2 plus TGFβ and RA. Total RNA was isolated from 2 × 10⁶ cells, and then labeled and hybridized to microarray chips for gene profiling. 32 representative genes display differential gene expression were shown (A). dChip software using FC > 2 and p < 0.05 identified unique genes that were exclusive either Foxp3 expression (B). Signal values for genes relevant to cytotoxic function (C) Treg stability (D) suppressive mechanisms (E) or Treg plasticity were displayed (F). * p < .05; **p < .01; ***p < .001. Error bars indicate the mean of standard error.