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. 2016 Jul 8;11(7):e0159191. doi: 10.1371/journal.pone.0159191

Fig 1. APL improved palmitate -induced insulin resistance in skeletal muscle myotubes.

Fig 1

(A) Differentiated L6 cells were pretreated with palmitate (PA,0.75 mM) for 16 h, then incubated with 1, 5 or 10 μM APL for 24 h in the presence or absence of insulin (100 nM). Cells were collected and 2-NBDG glucose uptake was assessed. (B) Differentiated L6 cells were pretreated with palmitate (PA,0.75 mM) for 16 h, then incubated with APL (10 μM) for 6, 12 or 24 h in the presence or absence of insulin (100 nM). Cells were collected and 2-NBDG glucose uptake was assessed. (C) Differentiated L6 cells were untreated or pretreated with palmitate (PA,0.75 mM) for 16 h, then incubated with 10 μM APL for 24 h in the presence or absence of insulin (100 nM). IRS-1, p-IRS-1, Akt, and p-Akt were detected by western blot. (D) Cells were treated as described in (A). IRS-1, p-IRS-1, Akt, and p-Akt were detected by western blot. Values are means ± SEM. n = 3, ap < 0.05 versus palmitate -treated group. A. U., arbitrary units. All results are representative western blots of three independent experiments with similar results.