Table 1. Details of the primers pairs used for RT-qPCR.
| Gene name (abbreviation) | Accession number | Primer Sequence (5’-3’) | Amplicon length (bp) | Tm (°C) | Efficiency (%) | R2 |
|---|---|---|---|---|---|---|
| Actin (ACT) | KU664390 | F: GAGCCGTCTTTCCATCCAT | 192 | 59.80 | 85.1 | 0.990 |
| R: TGTAGAAGGTGTGATGCCAGAT | ||||||
| Beta-1-tubulin (TUB) | KU664388 | F: GCACCCTCCTCATCTCCAAG | 209 | 60.10 | 103.7 | 0.998 |
| R: GAGTTTGAGCGTGCGGAAG | ||||||
| 18S ribosomal (18S) | JN036435 | F: CTGAGAAACGGCTACCACATC | 204 | 59.15 | 101.0 | 0.999 |
| R: GCTATTGGAGCTGGAATTACC | ||||||
| 60S ribosomal protein L13 a (RPL13A) | KU664391 | F: CATTTCAGAGCACCATCCAAG | 236 | 59.10 | 105 | 0.993 |
| R: CGATACTTCCAGCCAACTTCAT | ||||||
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | KU664394 | F: TTAGTCGTCAATGGCAACAAG | 207 | 59.02 | 87.2 | 0.990 |
| R: GACACCGACGACGAACATAG | ||||||
| Glucose-6-phosphate 1-dehydrogenase (G6PDH) | KU664393 | F: TGACACCCACTTTCGCCT | 237 | 59.15 | 102.5 | 0.990 |
| R: GTCTTGCCTGGTGCTTTACA | ||||||
| Translation elongation factor 2 (EF) | KU664389 | F: TCGGGAGAACACATCATTGC | 221 | 59.65 | 101.5 | 0.999 |
| R: GGAAGACCGTCGGGCATAG | ||||||
| Translation initiation factor 4A transporter-like (EIF4A) | KU664392 | F: GGAATGGAACCTGATGGAGT | 188 | 59.20 | 101.9 | 1.000 |
| R: GACTGAGCCTGAGCAATGAC |