Table 3. Ranking of candidate reference genes based on BestKeeper, geNorm and NormFinder analyses.
Biotic conditions | Bestkeeper | geNorm | NormFinder |
---|---|---|---|
Developmental stage | EIF4A | EIF4A/GAPDH | EF |
GAPDH | TUB | ||
EF | TUB | G6PDH | |
TUB | EF | EIF4A | |
RPL13A | G6PDH | RPL13A | |
G6PDH | RPL13A | GAPDH | |
ACT | ACT | ACT | |
18S | 18S | 18S | |
Body parts | EF | EIF4A/GAPDH | EF |
EIF4A | ACT | ||
GAPDH | EF | RPL13A | |
TUB | ACT | GAPDH | |
ACT | RPL13A | EIF4A | |
RPL13A | G6PDH | G6PDH | |
18S | 18S | 18S | |
G6PDH | TUB | TUB | |
Low temperature | EIF4A | EF/ RPL13A | TUB |
RPL13A | EIF4A | ||
EF | GAPDH | G6PDH | |
TUB | G6PDH | RPL13A | |
GAPDH | TUB | ACT | |
G6PDH | EIF4A | EF | |
18S | ACT | GAPDH | |
ACT | 18S | 18S | |
Fungi infection | GAPDH | G6PDH/18S | EF |
EF | 18S | ||
TUB | EIF4A | TUB | |
ACT | EF | EIF4A | |
18S | TUB | G6PDH | |
G6PDH | GAPDH | GAPDH | |
EIF4A | ACT | RPL13A | |
RPL13A | RPL13A | ACT | |
Diets | ACT | EF/GAPDH | ACT |
TUB | EF | ||
EF | TUB | EIF4A | |
EIF4A | EIF4A | TUB | |
G6PDH | RPL13A | RPL13A | |
GAPDH | ACT | GAPDH | |
RPL13A | G6PDH | G6PDH | |
18S | 18S | 18S |