Fig 1. PTH1 receptor levels increase in islets of PPx mice.

(A) PPx experimental design for analysis of glucose and beta cell homeostasis. Eight week-old male Balb/c mice underwent PPx or sham-operation at day 0, and were injected s.c. for 5 days/week with vehicle (veh) or PTHrP(1–36) (P36) at 160μg/kg body weight, resulting in four groups of mice: sham-veh, sham-PTHrP, PPx-veh, and PPx-PTHrP. The four groups of mice were treated for 7 days (n = 5 mice/group), 30 days (n = 4 mice/group), or 90 days (n = 6 mice/group). IPGTT was performed at days 25 and 79 (solid arrows) and ITT at days 16 and 74 (dotted arrows) on the four groups of mice. Representative western blot analysis of (B) PTHrP, and (D) PTH1R, with tubulin as loading control, in islets isolated from mice treated for 7 days. Western blot analysis was performed on a separate additional group of mice from those shown in Fig 1A. Quantification of the expression of (C) PTHrP/tubulin, and (E) PTH1R/tubulin ratios in the four groups of mice: sham-veh (black bars), sham-PTHrP (stippled bars), PPx-veh (grey bars) and PPx-PTHrP (horizontally-stippled bars) (n = 9–12 mice/group). Significance of * p<0.02 by ANOVA versus corresponding sham controls.